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dc.contributor.advisorShydlovska, Olga-
dc.contributor.authorDong, Haonan-
dc.date.accessioned2023-08-24T21:25:07Z-
dc.date.available2023-08-24T21:25:07Z-
dc.date.issued2023-
dc.identifier.citationHaonan Dong. Separation and screening of Acetobacter producing alcohol dehydrogenase : Bachelor’s thesis. Speciality 162 "Biotechnology and Bioengineering" / Haonan Dong ; scientific supervisor Olga Shydlovska ; reviewer Ihor Hretskyi. – Kyiv : KNUTD, 2023. – 40 p.uk
dc.identifier.urihttps://er.knutd.edu.ua/handle/123456789/24325-
dc.description.abstractIn mammals, alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) constitute the main oxidation channels of ethanol metabolism, which are the main limiting factors of ethanol metabolism in the body. ADH mainly catalyzes the production of acetaldehyde from ethanol and is the first step in the oxidized process of ethanol in various organs and tissues of humans and animals. Therefore, it is of great significance in relieving alcoholism and preventing liver damage caused by ethanol. Alcohol dehydrogenase has been found in animals, plants, microorganisms, eukaryotes, and prokaryotic bacteria, which is widely presented in microorganisms. Modern research has proven that the brewing of vinegar is carried out by cell membranes of acetic acid bacteria binding enzymes that complete acetic acid fermentation through the oxidation of alcohol-by-alcohol dehydrogenase and the oxidation of acetaldehyde-by-acetaldehyde dehydrogenase. The fermentative ability of acetic acid bacteria is related to the activity of these two enzymes, especially the former. Therefore, isolating strains with high ADH production from vinegar is of great research which is significant for establishing a probiotic library for detoxifying alcohol and protecting the liver. This article enriched and screened well-known vinegar mash samples in China, and obtained Acetobacter D2 with ADH activity reaching 4.288×10-2U. Furthermore, its activity was improved to 8.254×10-2U through UV mutagenesis. The object of the work is Acetobacter D2 with ADH activity. The subject of the work is ADH activity of isolated from vinegar Acetobacter D2. The aim of the work is screen and isolate effective Acetobacter produced alcohol dehydrogenase. The tasks of the work are to isolate from vinegar ADH active strain of Acetobacter, obtain the ADH activity, and improve ADH activity with UV mutagenesis.uk
dc.language.isoenuk
dc.publisherКиївський національний університет технологій та дизайнуuk
dc.subjectAlcohol dehydrogenaseuk
dc.subjectAcetobacteruk
dc.subjectscreeninguk
dc.subjectUV mutagenesisuk
dc.subjectenzymeuk
dc.titleSeparation and screening of Acetobacter producing alcohol dehydrogenaseuk
dc.typeДипломний проектuk
local.subject.facultyФакультет хімічних та біофармацевтичних технологійuk
local.subject.departmentКафедра біотехнології, шкіри та хутраuk
local.subject.method1uk
local.diplom.groupBEBT-19uk
local.diplom.okrБакалаврuk
local.diplom.speciality162 Biotechnology and Bioengineeringuk
local.diplom.programBiotechnologyuk
Розташовується у зібраннях:Кафедра біотехнології, шкіри та хутра (БШХ)
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