Cloning and analysis of CPN60 gene of grape molecular chaperone

Abstract

Grapes are widely cultivated fruit trees in the world, rich in antioxidant, anti-aging and cardiovascular disease prevention health function substances such as resveratrol and anthocyanins, and have a broad consumer market and huge economic value, and grape production is also restricted by various factors, so how to improve grape yield is also of great research significance. In all the life activities of grape, photosynthesis is closely related to the improvement of grape yield. Previous studies have shown that there is a molecular chaperone Cpn60 in plants that can use the hydrolysis of ATP to help new and formed or immature peptides complete the folding assembly, thus participating in the folding equipment process of proteins that play a key role in photosynthesis, and thus affecting the efficiency of photosynthesis. Therefore, in this study, CPN60 gene of different varieties of grapes was cloned, and amino acid sequence analysis of the protein encoded by this gene, protein structure prediction, protein physicochemical action analysis, protein interaction and exploration of its biological function were carried out.