Determination of the composition of sapons in biotransformed medicinal raw material of Gynostemma pentaphyllum (Thunb.) Makino

Abstract

Gynostema pentaphyllum is a traditional Chinese herb. Gypenosides are one of its main active ingredients, and their structure is a tetracyclic saponin of the Damaran type. Saponins of G. pentaphyllum reduce blood sugar and blood lipids, have antitumor, anti-inflammatory, antiviral and antifungal effects, increase immunity and protect the liver. Resources of G. pentaphyllum in China are very rich, proven sufficient resource potential as an imported raw material and the possibility of cultivation in the climatic conditions of Ukraine. The components and contents of gypenosides from different producing areas and different harvesting periods were different. The greatest accumulation occurred during the flowering period of the plant. Gypenosides were transformed by saponinase-assisted extraction and ethanol extraction and pectinase-assisted extraction, and the degree of transformation was different for gypenosides from different origins. When using the herb G. pentaphyllum in it is necessary to control the components and content of saponins in plant raw materials from different harvesting areas and the timing of harvesting.